Abstract |
BALB/c and C57BL/6J mice were immunized with recombinant vaccines consisting of lymphocytic choriomeningitis virus CD8+ T-lymphocyte epitopes and a carrier protein. During challenge infection with WE strain lymphocytic choriomeningitis virus, mutants with alterations in distinct amino acid residues of the epitopic nonapeptides appeared and multiplied. Splenocytes from WE-infected BALB/c mice lysed cells coated with the WE-type epitope; lysis was considerably less effective when the epitopic nonapeptide with which the syngeneic cells had been sensitized was the mutated form. Neither target was lysed by splenocytes from BALB/c mice infected with the variant virus. Mutants were not detected in F1 hybrid mice immunized with two viral epitopes that were restricted by class I molecules of both parents.
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Authors | G Weidt, W Deppert, O Utermöhlen, J Heukeshoven, F Lehmann-Grube |
Journal | Journal of virology
(J Virol)
Vol. 69
Issue 11
Pg. 7147-51
(Nov 1995)
ISSN: 0022-538X [Print] United States |
PMID | 7474135
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA Primers
- DNA, Complementary
- DNA, Viral
- Epitopes
- Vaccines, Synthetic
- Viral Core Proteins
- Viral Vaccines
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Topics |
- Amino Acid Sequence
- Animals
- Base Sequence
- CD8-Positive T-Lymphocytes
(immunology)
- Capsid
(chemistry, immunology)
- DNA Primers
- DNA, Complementary
- DNA, Viral
(analysis, chemistry)
- Epitopes
(immunology)
- Female
- Lymphocytic choriomeningitis virus
(genetics, immunology, isolation & purification)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Mice, Inbred Strains
- Molecular Sequence Data
- Mutagenesis
- Polymerase Chain Reaction
- Vaccines, Synthetic
- Viral Core Proteins
(chemistry, immunology)
- Viral Vaccines
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