Emergence of virus escape mutants after immunization with epitope vaccine.

Abstract

BALB/c and C57BL/6J mice were immunized with recombinant vaccines consisting of lymphocytic choriomeningitis virus CD8+ T-lymphocyte epitopes and a carrier protein. During challenge infection with WE strain lymphocytic choriomeningitis virus, mutants with alterations in distinct amino acid residues of the epitopic nonapeptides appeared and multiplied. Splenocytes from WE-infected BALB/c mice lysed cells coated with the WE-type epitope; lysis was considerably less effective when the epitopic nonapeptide with which the syngeneic cells had been sensitized was the mutated form. Neither target was lysed by splenocytes from BALB/c mice infected with the variant virus. Mutants were not detected in F1 hybrid mice immunized with two viral epitopes that were restricted by class I molecules of both parents.

• Authors: G Weidt, W Deppert, O Utermöhlen, J Heukeshoven, F Lehmann-Grube
• Journal: Journal of virology (J Virol) Vol. 69 Issue 11 Pg. 7147-51 (Nov 1995) ISSN: 0022-538X [Print] United States
• PMID: 7474135 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• DNA, Complementary
• DNA, Viral
• Epitopes
• Vaccines, Synthetic
• Viral Core Proteins
• Viral Vaccines

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• CD8-Positive T-Lymphocytes (immunology)
• Capsid (chemistry, immunology)
• DNA Primers
• DNA, Complementary
• DNA, Viral (analysis, chemistry)
• Epitopes (immunology)
• Female
• Lymphocytic choriomeningitis virus (genetics, immunology, isolation & purification)
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• Mice, Inbred Strains
• Molecular Sequence Data
• Mutagenesis
• Polymerase Chain Reaction
• Vaccines, Synthetic
• Viral Core Proteins (chemistry, immunology)
• Viral Vaccines