Adenovirus 12
mRNA's transcribed from the transforming region were analyzed and mapped on
viral DNA by the nuclease S1 gel and
diazobenzyloxymethyl paper blot techniques in cells transformed of adenovirus 12
DNA and in cells early and late after lytic
infection with adenovirus 12. Two initiation sites of
mRNA transcription and two kinds of splicing were found in each of early regions 1A and 1B. For early region 1A
mRNA's, four species were found in lytically infected cells. Three of them were commonly found in cells transformed by either HindIII-G or EcoRI-C. Cells transformed by HindIII-G contained two additional 1A transcripts, which could be the 3' portions of chimeric
mRNA's of cellular-viral or viral-viral sequences. Transcription in the 1B region diverged among the above cell lines. Early after lytic
infection, no appreciable amount of 1B
mRNA was detected, whereas two species of
mRNA's, one corresponding to
protein IX
mRNA and another having splicing, were found at the late stage. In cells transformed by EcoRI-C, a distinct
mRNA species with splicing was observed. Cells transformed by HindIII-G contained a transcript from the leftmost part of the 1B sequence at the 5' portion of chimeric
mRNA species, suggesting the presence of tandem integration of
viral DNA in the cells. Other
mRNA species in the 1A and 1B regions were also detected in both transformed cell lines. The results are discussed in relation to the nucleotide sequence of the HindIII-G fragment of adenovirus 12
DNA.