Abstract |
The effect of extracellular pH on hexose uptake by cultured Novikoff hepatoma cells has been examined with respect to four distinct parameters: i) the transport of 3-O-methyl-D-glucose, ii) the uptake of 2-deoxy-D-glucose by intact cells, iii) the phosphorylation of 2-deoxy-D-glucose in vitro by cell-free preparations, and iv) the intracellular pH as measured by 5,5'-dimethyl[2-14C] oxazolidine-2,4-dione. Transport per se was not affected by pH in the range of 6 to 8, while uptake of 2-deoxy-D-glucose increased with pH 2- to 3-fold over this range. The pH sensitivity of uptake can be explained on the basis of a change in hexokinase activity effective in situ. The pH dependence of hexokinase observed in vitro, however, was insufficient to account for that apparent in situ.
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Authors | R M Wohlhueter, P G Plagemann |
Journal | The Journal of biological chemistry
(J Biol Chem)
Vol. 256
Issue 2
Pg. 869-75
(Jan 25 1981)
ISSN: 0021-9258 [Print] United States |
PMID | 7451478
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Deoxy Sugars
- Methylglucosides
- Methylglycosides
- Deoxyglucose
- Hexokinase
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Topics |
- Animals
- Biological Transport, Active
- Deoxy Sugars
(metabolism)
- Deoxyglucose
(metabolism)
- Hexokinase
(metabolism)
- Hydrogen-Ion Concentration
- Kinetics
- Liver Neoplasms, Experimental
(metabolism)
- Methylglucosides
(metabolism)
- Methylglycosides
(metabolism)
- Phosphorylation
- Rats
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