Purification and characterization of a lipolytic factor (toxohormone-L) from cell-free fluid of ascites sarcoma 180.

Abstract

A lipolytic substance in the ascites fluid of mice with Sarcoma 180, called toxohormone-L, was purified and characterized. The lipolytic activity of toxohormone-L was measured in vitro using rat adipose tissue slices. Toxohormone-L, purified approximately 90-fold from the ammonium sulfate fraction, gave a single band on polyacrylamide gel electrophoresis. Its molecular weight was about 75,000, and its isoelectric point was 4.7. Toxohormone-L is heat labile and nondialyzable, but, on its digestion with trypsin, an active fragment that was heat stable and dialyzable was produced. Toxohormone-L is a protein and is also present in the ascites fluid of patients with hepatoma and Grawitz's tumor.

• Authors: H Masuno, N Yamasaki, H Okuda
• Journal: Cancer research (Cancer Res) Vol. 41 Issue 1 Pg. 284-8 (Jan 1981) ISSN: 0008-5472 [Print] United States
• PMID: 7448767 (Publication Type: Journal Article)

Chemical References

• Endotoxins
• Neoplasm Proteins
• Triglycerides
• toxohormone

Topics

• Animals
• Endotoxins (isolation & purification)
• Exudates and Transudates (analysis)
• Isoelectric Point
• Lipolysis
• Mice
• Molecular Weight
• Neoplasm Proteins (isolation & purification)
• Sarcoma, Experimental (analysis)
• Triglycerides (metabolism)