Abstract |
A subunit (designated B1) of the tumor associated peptide " Tissue Polypeptide Antigen" (TPA) has been cleaved by cyanogen bromide. Some of the fragments contained TPA-activity (as measured by a standardized hemagglutination inhibition method). The fragments have been separated, and partial amino acid sequences have been determined for some of the fragments. A method for purification of the most active fragment (designated BrCN:C) has been worked out from cleavage products obtained by cleavage with cyanogen bromide of "Washed Tissue Powder". By this method fragment BrCN:C has been isolated from a pool of placentae and a pool or carcinoma tumors, and the amino acid sequences of more than 50 amino acids from the N-terminus have been determined. Fragment BrCN:C has been demonstrated from three different sources, which indicates the presence of a common part in subunit B1 independent of source.
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Authors | P Redelius, B Lüning, B Björklund |
Journal | Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry
(Acta Chem Scand B)
Vol. 34
Issue 4
Pg. 265-73
( 1980)
ISSN: 0302-4369 [Print] Denmark |
PMID | 7435086
(Publication Type: Journal Article)
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Chemical References |
- Amino Acids
- Antigens, Neoplasm
- Peptide Fragments
- Peptides
- Tissue Polypeptide Antigen
- Cyanogen Bromide
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Topics |
- Amino Acid Sequence
- Amino Acids
(analysis)
- Antigens, Neoplasm
- Chemical Phenomena
- Chemistry
- Chromatography, High Pressure Liquid
- Cyanogen Bromide
- Humans
- Peptide Fragments
(analysis, isolation & purification)
- Peptides
- Tissue Polypeptide Antigen
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