Abstract |
Primary cultures of neonatal rat cardiac muscle cells incorporated radioiron from both [55Fe] transferrin and 59FeCl3 (added simultaneously). To evaluate the effect of iron chelators on such uptake, deferri chelators were added 6 hr after addition of the radioiron sources. The microbial chelator agrobactin was significantly more effective than the drug defoxamine in reduction of 55Fe uptake from [55Fe] transferrin; both chelators halted 59Fe3+ uptake. Agrobactin may have potential in chelation therpay for iron-overload disease. Certain other microbial chelators lowered radioiron uptake from either [55Fe] transferrin of 59FeCl3. These chelators should be useful inhibitors for studies of animal cell iron uptake and intracellular iron flow.
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Authors | C V Sciortino, B R Byers, P Cox |
Journal | The Journal of laboratory and clinical medicine
(J Lab Clin Med)
Vol. 96
Issue 6
Pg. 1081-5
(Dec 1980)
ISSN: 0022-2143 [Print] United States |
PMID | 7430764
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Iron Chelating Agents
- Iron Radioisotopes
- Deferoxamine
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Topics |
- Animals
- Deferoxamine
(therapeutic use)
- Iron Chelating Agents
(therapeutic use)
- Iron Radioisotopes
(metabolism)
- Myocardium
(metabolism)
- Rats
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