Biological activity of 1,N6-ethenothiamin, a fluorescent analog of thiamin, in rats.

Abstract

The ability of the recently synthesized fluorescent analog of thiamin, 1,N6-ethenothiamin, to substitute for thiamin in rats in vivo has been studied. 50 micrograms of ethenothiamin/100 g body weight/day injected in rats allowed about one-half the weight gain with 50 micrograms/100g/day of thiamin. The activities of the pyruvate and alpha-keto-glutarate dehydrogenases of liver mitochondria were partially restored by 50 micrograms/100g/day of ethenothiamin. Ethenothiamin at this level did not appear to restore the branched-chain alpha-keto acid dehydrogenase activities to any extent. When 150 micrograms of ethenothiamin/100g body weight/day was injected, growth was optimum, but these enzyme activities still did not achieve normal levels.

• Authors: W A Gibby, C J Gubler
• Journal: The Journal of nutrition (J Nutr) Vol. 110 Issue 10 Pg. 2117-20 (Oct 1980) ISSN: 0022-3166 [Print] United States
• PMID: 7420211 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Amino Acids, Branched-Chain
• Keto Acids
• Multienzyme Complexes
• ethenothiamin
• Ketone Oxidoreductases
• 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
• Thiamine

Topics

• 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
• Amino Acids, Branched-Chain (metabolism)
• Animals
• Body Weight (drug effects)
• Dose-Response Relationship, Drug
• Keto Acids (metabolism)
• Ketone Oxidoreductases (metabolism)
• Male
• Mitochondria, Liver (enzymology)
• Multienzyme Complexes (metabolism)
• Rats
• Structure-Activity Relationship
• Thiamine (analogs & derivatives, pharmacology)
• Thiamine Deficiency (metabolism)