Inhibition of dengue virus replication by amantadine hydrochloride.

Abstract

The effect of amantadine hydrochloride (1-adamantanamine hydrochloride) on dengue virus replication was examined in vitro. Amantadine decreased the titers of all four types of dengue viruses grown in LLC-MK2 cells by greater than 90% at concentrations of 50 micrograms/ml. There was no evidence for any cytopathic effect of the drug at concentrations less than 100 micrograms/ml. Studies of the time of addition showed that the antiviral effect was maximal when drug was added to virus cultures immediately after the viral adsorption period. In addition, amantadine caused a marked reduction in the growth of dengue virus type 2 in both human and rhesus peripheral blood leukocytes without affecting cell viabili ty. These findings demonstrate that amantadine significantly inhibits the replication of dengue viruses in vitro and indicate a need to determine the efficacy of this drug against dengue virus infections in vivo.

• Authors: W C Koff, J L Elm Jr, S B Halstead
• Journal: Antimicrobial agents and chemotherapy (Antimicrob Agents Chemother) Vol. 18 Issue 1 Pg. 125-9 (Jul 1980) ISSN: 0066-4804 [Print] United States
• PMID: 7416739 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Amantadine

Topics

• Amantadine (pharmacology)
• Animals
• Dengue (etiology)
• Dengue Virus (drug effects, immunology)
• Dose-Response Relationship, Drug
• Humans
• Leukocytes (metabolism)
• Macaca mulatta
• Time Factors
• Virus Replication (drug effects)