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Effects of a tumor promoter and an anti-promoter on spontaneous and UV-induced 6-thioguanine-resistant mutations and sister-chromatid exchanges in V79 Chinese hamster cells.

Abstract
The effects of a tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) and/or an anti-promoter antipain (protease inhibitor) on spontaneous and ultraviolet-induced sister-chromatid exchanges (SCEs) and 6-thioguanine-resistant (6TGr) recessive mutations were examined in V79 Chinese hamster cells in culture. TPA and/or antipain neither significantly altered base-line and UV-induced immediate SCE frequencies, nor decreased the level of delayed SCEs which persisted 6-7 days after irradiation. TPA and/or antipain appeared to enhance the recovery of UV-induced 6TGr colonies at the plateau expression phase despite non-mutagenicity by themselves and unaltered metabolic cooperation. Thus, the results conceivably imply that the 6TGr-recessive mutation expression, but not fixation, can be modulated at the cell level by TPA and/or antipain. Our results, together with the recent results of Loveday and Latt, may argue against the notion that TPA enhances the antipain-suppressible SCEs as an index of mitotic recombination in relevance with a tumor-promotion mechanism.
AuthorsY Fujiwara, Y Kano, M Tatsumi, P Paul
JournalMutation research (Mutat Res) Vol. 71 Issue 2 Pg. 243-51 (Jul 1980) ISSN: 0027-5107 [Print] Netherlands
PMID7393240 (Publication Type: Journal Article)
Chemical References
  • Oligopeptides
  • Phorbols
  • Antipain
  • Thioguanine
  • Tetradecanoylphorbol Acetate
Topics
  • Animals
  • Antipain (pharmacology)
  • Cell Line
  • Cricetinae
  • Cricetulus
  • Crossing Over, Genetic (drug effects)
  • Mutation
  • Oligopeptides (pharmacology)
  • Phorbols (pharmacology)
  • Sister Chromatid Exchange (drug effects, radiation effects)
  • Tetradecanoylphorbol Acetate (pharmacology)
  • Thioguanine (pharmacology)
  • Ultraviolet Rays

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