A simple procedure for the radioimmunoassay of 17 alpha-hydroxyprogesterone in serum: comparison with an immunological purification technique.

Abstract

A simple radioimmunoassay for the measurement of 17 alpha-hydroxyprogesterone in 0.1-0.25 ml serum is described. An antibody prepared against 17 alpha-hydroxyprogesterone 3(O-carboxymethyl)-oxime-BSA as immunogen was used. A correlation of the 17 alpha-hydroxyprogesterone concentration found in the serum of normal subjects and of patients with that obtained by a more complex immunological purification method was good (r=0.924, P less than 0.001). Accuracy, precision, and specificity were acceptable with the simple method. Normal values for infants, children, and adults have been established, and the individual concentrations of 17 alpha-hydroxyprogesterone in the serum of patients withcongenital adrenal hyperplasia were well separated from normal values.

• Authors: N Davila, B T Rudd, R Morris, F Kandeel, J Bodden, D R London
• Journal: Annals of clinical biochemistry (Ann Clin Biochem) Vol. 17 Issue 1 Pg. 31-7 (Jan 1980) ISSN: 0004-5632 [Print] England
• PMID: 7377742 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Hydroxyprogesterones
• Charcoal

Topics

• Adrenal Hyperplasia, Congenital (blood)
• Adult
• Charcoal
• Child
• Female
• Humans
• Hydroxyprogesterones (blood)
• Infant
• Male
• Radioimmunoassay (methods)
• Reference Values