Cellular estrogen and progesterone receptors in mammary carcinoma.

Abstract

Cellular estrogen and progesterone receptors of human mammary cancer in cryostat-frozen sections were studied simultaneously. The receptor tracers used were a mixture of 17 beta-estradiol-6-carboxymethyl oxime-bovine serum albumin-fluorescein isothiocyanate and 11 alpha-hydroxyprogesterone hemisuccinate-bovine serum albumin-tetramethylrhodamine isothiocyanate conjugate. After incubation with the tracers, the estrogen-binding sites manifested apple-green, and the progesterone-binding sites manifested orange-red fluorescence when examined with a fluorescence microscope equipped with appropriate filters. The findings indicate that most estrogen-receptor-positive epithelial cells, benign or malignant, of the mammary glands contain a significant number of progesterone-receptor sites. Incorporation of a fluorescent progesterone conjugate into the histochemical reagent may help to confirm the existence of an operative receptor system in the cancer cells.

• Authors: S H Lee
• Journal: American journal of clinical pathology (Am J Clin Pathol) Vol. 73 Issue 3 Pg. 323-9 (Mar 1980) ISSN: 0002-9173 [Print] England
• PMID: 7361711 (Publication Type: Journal Article)

Chemical References

• Receptors, Estrogen
• Receptors, Progesterone

Topics

• Adult
• Aged
• Breast Neoplasms (metabolism)
• Female
• Frozen Sections
• Histocytochemistry
• Humans
• Middle Aged
• Receptors, Estrogen (metabolism)
• Receptors, Progesterone (metabolism)