Prenatal detection of cystic fibrosis.

Abstract

Previous studies in our laboratory have suggested that measurement of methylumbelliferylguanidinobenzoate (MUGB) reactive proteases in midtrimester amniotic fluid is potentially of value for the intrauterine detection of cystic fibrosis. Thirty-nine pregnancies of obligate heterozygotes of cystic fibrosis and 12 cases where one parent has a sib with cystic fibrosis have been previously monitored by means of quantitative and qualitative measurements of MUGB in amniotic fluid. In all but one case the diagnosis was accurately ascertained in the midtrimester and confirmed after delivery. The measurement of MUGB reactivity in midtrimester amniotic fluid by three different procedures--quantitative analysis, polyacrylamide isoelectric focusing, and column filtration--appears to provide a practical and reliable approach for the intrauterine detection of cystic fibrosis.

• Authors: H L Nadler, P Rembelski, K Meserow, A B Gerbie
• Journal: American journal of obstetrics and gynecology (Am J Obstet Gynecol) Vol. 141 Issue 8 Pg. 885-9 (Dec 15 1981) ISSN: 0002-9378 [Print] United States
• PMID: 7315920 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• 4-methylumbelliferylguanidinobenzoate
• Hymecromone

Topics

• Amniotic Fluid (analysis)
• Cystic Fibrosis (diagnosis, genetics)
• Female
• Heterozygote
• Humans
• Hymecromone (analogs & derivatives, analysis)
• Pregnancy
• Pregnancy Trimester, Second
• Prenatal Diagnosis