A rapid and simple method for the determination of picogram levels of 3-methoxytyramine in brain tissue using liquid chromatography with electrochemical detection.

Abstract

A rapid and simple technique using solvent extraction, ion-pairing extraction, and high pressure liquid chromatography with electrochemical detection has been developed for the determination of 3-methoxytyramine in striata of rats killed by microwave irradiation. The method is specific and reproducible (coefficient of variation among replications, +/- 4%); recovery of authentic 3-methoxytyramine added to the samples is 45-50%. 3-Methoxytyramine levels found with this technique in rat striata were 15 +/- 1.7 ng/g. The method has a sensitivity of about 0.2 pmol per brain sample. Monoamine oxidase inhibition with pargyline increased 3-methoxytyramine levels in rat striata, while catechol-O-methyltransferase inhibition with 3',4'-dihydroxy-2 methylpropiophenone completely depleted 3-methoxytyramine. The effects of nomifensine, quipazine, caroxazone, piribedil, and D-amphetamine were also examined. The 3-methoxytyramine concentrations in the brains of animals killed by decapitation or by microwave irradiation were compared.

• Authors: F Ponzio, G Achilli, S Algeri
• Journal: Journal of neurochemistry (J Neurochem) Vol. 36 Issue 4 Pg. 1361-7 (Apr 1981) ISSN: 0022-3042 [Print] England
• PMID: 7264632 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 3-methoxytyramine
• Dopamine

Topics

• Animals
• Brain Chemistry
• Chromatography, High Pressure Liquid (methods)
• Corpus Striatum (analysis, drug effects)
• Dopamine (analogs & derivatives, analysis, metabolism)
• Electrochemistry
• Male
• Microchemistry
• Microwaves
• Postmortem Changes
• Rats