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Level of methionine synthase activity and interconversion of methylcobalamin and adenosylcobalamin in a facultative methylotroph, Protaminobacter ruber.

Abstract
Protaminobacter ruber was cultured in a medium containing [57Co]cyanocobalamin with a "two-step cultivation method" and the forms of vitamin B12 compounds in the cells were examined. Methylcobalamin was detected in the early phases of growth and reached a maximum of about 40% of all cobalamins extracted from the cells. In the stationary phase of growth, almost all cobalamins consisted of adenosylcobalamin. Recultivation of the cells of the stationary phase in a fresh medium resulted in the conversion of adenosylcobalamin into methylcobalamin. Interconversion of methylcobalamin and adenosylcobalamin was presumed from these facts. The formation of adenosylcobalamin from methylcobalamin was demonstrated with a cell-free extract system from P. ruber. The rate of conversion of methylcobalamin into adenosylcobalamin was highest among several cobalamin analogs tested. Propylation of 5-methyltetrahydrofolate: homocysteine methyltransferase with 1-iodopropane did not affect this conversion reaction, which was probably catalyzed by methyltransferase and adenosyltransferase.
AuthorsK Sato, S Shimizu
JournalJournal of nutritional science and vitaminology (J Nutr Sci Vitaminol (Tokyo)) Vol. 26 Issue 6 Pg. 557-69 ( 1980) ISSN: 0301-4800 [Print] Japan
PMID7241237 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Cobamides
  • mecobalamin
  • Methyltransferases
  • 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
  • cobamamide
  • Vitamin B 12
Topics
  • 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase (analysis)
  • Cobamides (metabolism)
  • Methyltransferases (analysis)
  • Pseudomonadaceae (enzymology, growth & development, metabolism)
  • Time Factors
  • Vitamin B 12 (analogs & derivatives, metabolism, pharmacology)

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