Metabolism of 3,3'-Diiodothyronine and 3'-monoiodothyronine, and effect of potassium cyanide and dinitrophenol and glucuronidation of thyroxine in cultured rat hepatoma cells.

Abstract

Rat hepatoma cells (R117-21B) metabolized 3,3'diiodothyronine and 3'-monoiodothyronine by sulfation and glucuronidation. Glucuronidation of iodothyronines (a mixture of 3,3'-diiodothyronine, approximately 99%, and 3'-monoiodothyronine, approximately 10%(was very rapid and 83% of the total 125I in the medium was their glucuronides after 8 hr incubation with the cells at 37 degrees C. Iodothyronine sulfates (approximately 9% of the total 125I) were also detected. No clear increase in free iodide was observed during the 8 hr incubation. The effect of KCN or dinitrophenol on glucuronidation of thyroxine was investigated. Both drugs inhibited glucuronidation, and dinitrophenol was more effective than KCN. Dinitrophenol accelerated the liberation of 125I- in the metabolism of thyroxine in the cultured rat hepatoma cells.

• Authors: K Sorimachi, A Niwa, Y Yasumura
• Journal: Endocrinologia japonica (Endocrinol Jpn) Vol. 27 Issue 5 Pg. 631-6 (Oct 1980) ISSN: 0013-7219 [Print] Japan
• PMID: 7238420 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cyanides
• Diiodothyronines
• Dinitrophenols
• Glucuronates
• Thyronines
• 3'-monoiodothyronine
• Potassium Cyanide
• Thyroxine

Topics

• Animals
• Cells, Cultured
• Cyanides (pharmacology)
• Diiodothyronines (metabolism)
• Dinitrophenols (pharmacology)
• Glucuronates (metabolism)
• Liver Neoplasms, Experimental (metabolism)
• Potassium Cyanide (pharmacology)
• Rats
• Thyronines (metabolism)
• Thyroxine (metabolism)