Abstract |
The cellular localization of the molecular forms of acetylcholinesterase was explored in chick sympathetic neurons and in mouse T28 cells ( neuroblastoma X sympathetic ganglion cell hybrids) using the reversible, poorly lipid-soluble inhibitor of acetylcholinesterase, BW284C51, to protect cell surface activity while inactivating cytoplasmic activity with DFP, an irreversible, lipid-soluble inhibitor. Our results show protection of over 80% of the chick 11 S form and over 90% of the corresponding mouse 10 S form under these conditions, while over 90% of the chick 6.5 S and mouse 4 S forms are inhibited. The results suggest that the avian 11 S and mouse 10 S forms are predominantly or exclusively ectoenzymes while the respective 6.5 S and 4 S forms are confined to the cytoplasm.
|
Authors | P B Taylor, F Rieger, M L Shelanski, L A Greene |
Journal | The Journal of biological chemistry
(J Biol Chem)
Vol. 256
Issue 8
Pg. 3827-30
(Apr 25 1981)
ISSN: 0021-9258 [Print] United States |
PMID | 7217057
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- Isoenzymes
- Acetylcholinesterase
|
Topics |
- Acetylcholinesterase
(analysis)
- Animals
- Cell Line
- Cells, Cultured
- Chickens
- Ganglia, Sympathetic
(enzymology)
- Hybrid Cells
(enzymology)
- Isoenzymes
(analysis)
- Mice
- Molecular Weight
- Neuroblastoma
- Neurons
(enzymology)
|