Twenty-four to ninety-six-hour storage of human spermatozoa in test-yolk buffer.

Abstract

The feasibility of a simple, inexpensive technique of short-term (24- to 96-hour) preservation of human spermatozoa in TEST-yolk buffer is demonstrated. Preservation of spermatozoa for up to 72 hours would require no addition of antibiotics. There is little loss of motility when semen is added to TEST-yolk buffer and kept in a standard refrigerator. Such semen would then be available for multiple inseminations during the periovulatory period. One objection to this procedure is that it is necessary to dilute semen 1:1 in TEST-yolk buffer, resulting in a 50% lower sperm density, and this may be objectionable when oligospermia is present. Results obtained in this study are in agreement with similar studies performed on domestic animals.

• Authors: D G Jaskey, M R Cohen
• Journal: Fertility and sterility (Fertil Steril) Vol. 35 Issue 2 Pg. 205-8 (Feb 1981) ISSN: 0015-0282 [Print] United States
• PMID: 7202744 (Publication Type: Journal Article)

Chemical References

• Buffers
• Penicillins
• Streptomycin

Topics

• Adult
• Aerobiosis
• Anaerobiosis
• Buffers
• Humans
• Male
• Penicillins (pharmacology)
• Preservation, Biological
• Sperm Motility
• Spermatozoa (physiology)
• Streptomycin (pharmacology)
• Time Factors