Abstract |
To study the possibility that liver metabolism of acrylonitrile ( ACN) could produce a stable carcinogenic metabolite that causes the brain tumors produced by ACN, the ability of hepatocytes to generate a stable transportable mutagenic metabolite in culture was studied. ACN did not produce sister chromatid exchanges (SCEs) in Chinese hamster ovary (CHO) cells, but a significant increase was produced when CHO cells were co-cultured with freshly isolated rat hepatocytes. Induction of SCE in CHO cells co-cultured with hepatocytes suggests that the liver cells metabolize ACN and the reactive metabolite is transported from them to the CHO cells.
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Authors | S V Brat, G M Williams |
Journal | Cancer letters
(Cancer Lett)
1982 Nov-Dec
Vol. 17
Issue 2
Pg. 213-6
ISSN: 0304-3835 [Print] Ireland |
PMID | 7165873
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Acrylonitrile
(metabolism, pharmacology)
- Animals
- Biological Transport
- Biotransformation
- Cell Line
- Cricetinae
- Cricetulus
- Crossing Over, Genetic
(drug effects)
- Female
- Liver
(metabolism)
- Nitriles
(pharmacology)
- Ovary
- Rats
- Sister Chromatid Exchange
(drug effects)
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