A quantitative cytochemical study of acid phosphatases in hepatocytes of different ploidy classes from aging rats.

Abstract

A quantitative cytochemical study of isolated hepatocytes from young, middle-aged and old adult rats has shown a doubling of acid naphthol AS-BI phosphatase activity as the hepatocytes shift from the diploid to the tetraploid state, and an overall increase in activity in cells from old animals. There was a complete inhibition of all acid phosphatase activity with 10(-2)M sodium molybdate, but only a 90% inhibition in hepatocytes from livers of rats of 12-25 months-old animals with 10(-2)M sodium fluoride, the resistant activity possibly representing adenyl cyclase activity. In contrast, 10(-2)M ouabain inhibited 70-80% of the activity in hepatocytes from 12-15-months-old animals, this level of inhibition decreasing with increasing age, and implying a decrease in transport ATPase activity and an increased lysosomal phosphatase activity with increasing age of the rats.

• Authors: J Middleton, P B Gahan
• Journal: Experimental gerontology (Exp Gerontol) Vol. 17 Issue 4 Pg. 267-72 ( 1982) ISSN: 0531-5565 [Print] England
• PMID: 7160444 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ouabain
• Sodium Fluoride
• Acid Phosphatase

Topics

• Acid Phosphatase (antagonists & inhibitors, metabolism)
• Aging
• Animals
• Female
• Liver (cytology, enzymology)
• Ouabain (pharmacology)
• Ploidies
• Rats
• Rats, Inbred BN
• Sodium Fluoride (pharmacology)