[Protein assay for cellular receptors of steroids: a critical study of the technic with Coomassie-blue].

Abstract

The Coomassie-Blue method for protein determination is the EORTC uniformed technique for steroid receptor assay in tumor tissues. Many quality control trials have shown unacceptable interlaboratory variation coefficients for protein determination in steroid receptor assay. The objective of this study is to check the critical parameters of the method. The authors propose some suggestions on stability of dye-protein complex, ionic strength and temperature influence to improve the accuracy of the method.

• Authors: D Pelizzola, M De Bortoli, G Scaglianti, G Giovannini, S Fumero, A Piffanelli
• Journal: Bollettino della Societa italiana di biologia sperimentale (Boll Soc Ital Biol Sper) Vol. 58 Issue 21 Pg. 1432-8 (Nov 15 1982) ISSN: 0037-8771 [Print] Italy
• Vernacular Title: Dosaggio delle proteine nella determinazione dei recettori cellulari degli steroidi: studio critico della tecnica con Coomassie-blue.
• PMID: 7159536 (Publication Type: English Abstract, Journal Article)

Chemical References

• Receptors, Steroid
• Rosaniline Dyes
• coomassie Brilliant Blue

Topics

• Animals
• Methods
• Osmolar Concentration
• Receptors, Steroid (analysis)
• Rosaniline Dyes
• Spectrophotometry
• Temperature