Abstract |
The ability of 1,6-dinitropyrene and 1,8-dinitropyrene to induce chromosome aberrations has been examined in a rat epithelial cell line (RL4). Both compounds were found to be potent clastogenic agents in these cells, inducing predominantly chromatid type abberations. The number of aberrant metaphases was dose-related up to 1.25 micrograms/ml, after which the response showed a plateau at a frequency of around 60%. The number of chromatid gaps per 100 cells also increased linearly with concentration from 3.5 in the control cultures up to 117 with 1,6-DNP (1.25 micrograms/ml) and 98 with 1,8-DNP (2.5 micrograms/ml), indicating that the induction of chromatid gaps was a valid and sensitive measure of chromosome damage in this cell line. The fact that a positive result as obtained in RL4 cells implies that these cells contain enzyme systems which are capable of converting dinitropyrenes to a mutagenic form.
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Authors | N Danford, P Wilcox, J M Parry |
Journal | Mutation research
(Mutat Res)
Vol. 105
Issue 5
Pg. 349-55
(Nov 1982)
ISSN: 0027-5107 [Print] Netherlands |
PMID | 7144792
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Dinitrophenols
- Mutagens
- Pyrenes
- 1,8-dinitropyrene
- 1,6-dinitropyrene
- 2,4-Dinitrophenol
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Topics |
- 2,4-Dinitrophenol
- Animals
- Cell Line
- Chromosome Aberrations
- Dinitrophenols
(pharmacology)
- Epithelium
(drug effects)
- Kinetics
- Liver
(drug effects)
- Mutagenicity Tests
- Mutagens
- Mutation
- Pyrenes
(pharmacology)
- Rats
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