Abstract |
[14C] ethidium bromide has been used to determine drug levels in tissues and body fluids of rabbits and calves following intramuscular injection. Uninfected and Trypanosoma brucei- or Trypanosoma congolense-infected animals were studied. Blood and tissue fluid level reached a maximum with 1 h and then fell rapidly; after 96 h 80-90% of the radioactivity injected had been excreted, approximately one third in urine and two thirds in faeces. By 1 h after injection of 1 mg [14C] ethidium/kg into a T. congolense-infected calf, 70-80% of the radioactivity in blood was found to be bound to trypanosomes. Doses of 1 or 10 mg/kg were found not to be curative for T congolense or T. brucei infections in rabbits: drug treatment resulted in a period of sub-patent parasitaemia which was always followed by a relapse. Examination of the prophylactic action of ethidium in rabbits showed that the drug extended the pre-patent period following trypanosome inoculation but provided no absolute protection. A period of "apparent' prophylaxis observed after drug treatment of infected rabbits has been correlated with the presence of anti-trypanosome IgG in the serum.
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Authors | R J Gilbert, B A Newton |
Journal | Parasitology
(Parasitology)
Vol. 85 (Pt 1)
Pg. 127-48
(Aug 1982)
ISSN: 0031-1820 [Print] England |
PMID | 7122121
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Body Fluids
(metabolism)
- Cattle
- Ethidium
(blood, metabolism, therapeutic use)
- Feces
(analysis)
- Rabbits
- Tissue Distribution
- Trypanosoma
- Trypanosoma brucei brucei
- Trypanosomiasis, African
(drug therapy, metabolism)
- Trypanosomiasis, Bovine
(drug therapy, metabolism)
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