The effects of lesioning monoamine pathways in the rat brain on
tremorine-induced hind-
limb tremor and rigidity were studied. Nigro-striatal and mesolimbic
dopamine (DA) neurones were lesioned unilaterally by injecting
6-hydroxydopamine (6-OHDA) into the median forebrain bundle.
Tremor was reduced in the contralateral leg and rigidity was prevented in the ipsilateral leg. Injection of
6-OHDA into the nucleus accumbens affected
tremor but not rigidity. In general, nigral DA neurones may influence rigidity whilst mesolimbic DA neurones affect
tremor. A unilateral locus coeruleus electrolesion which destroys
noradrenaline (NA) fibres reduced both
tremor and rigidity. A median raphe electrolytic lesion affecting
5-hydroxytryptamine (5-HT) neurones had no effect on
tremor and rigidity, whereas lesioning the dorsal raphe electrolytically or by injecting
5,6-dihydroxytryptamine prevented rigidity without affecting
tremor. Electrical stimulation of the dorsal raphe increased transiently the hindlimb tone of normal rats. The findings demonstrate that the monoamines, especially
5-HT, are differently involved in the mechanisms of
tremor and rigidity produced by
tremorine.