Abstract |
Current responses to acetylcholine (ACh) and to suberyldicholine (D-6) applied from the double-barrelled ionophoretic micropipette were studied in two identified neurones (LPed-2 and LPed-3) isolated from the left ganglion of pulmonate mollusc, Planorbarius corneus. Experiments made with K2SO4-filled microelectrodes show that in LPed-2 neurone two kinds of cholinoreceptors are involved in the rapid phase of ACh response one of which induces chloride conductance and the other, sodium conductance. The Cl-dependent component can be separated from the cationic one by C-6 whereas the cationic component can be separated from the Cl--dependent one by furosemide. Cl- conductance can be induced selectively by D-6. In the LPed-3 neurone only Cl- conductance increases during rapid phase of ACh response. The reversal potential of Cl--dependent responses was found to be more negative than the resting potential in experiments made with K2SO4-filled microelectrodes but less negative than the resting potential in the case of KCl-filled microelectrodes. This difference seems to be due to the artificial increase of intracellular chloride concentration.
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Authors | A N Katchman, E V Zeimal |
Journal | Brain research
(Brain Res)
Vol. 241
Issue 1
Pg. 95-103
(Jun 03 1982)
ISSN: 0006-8993 [Print] Netherlands |
PMID | 7104710
(Publication Type: Journal Article)
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Chemical References |
- Chlorides
- Receptors, Cholinergic
- Receptors, Nicotinic
- subecholine
- Furosemide
- Sodium
- Choline
- Acetylcholine
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Topics |
- Acetylcholine
(pharmacology)
- Animals
- Chlorides
(pharmacology)
- Choline
(analogs & derivatives, pharmacology)
- Electric Conductivity
- Furosemide
(pharmacology)
- Kinetics
- Membrane Potentials
(drug effects)
- Mollusca
(physiology)
- Neurons
(physiology)
- Receptors, Cholinergic
(physiology)
- Receptors, Nicotinic
(drug effects, physiology)
- Sodium
(pharmacology)
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