Abstract |
The DNA content of individual nuclei in bladder washings was estimated by means of flow cytometry. A method of preparation using a detergent to produce single nuclei was used. By means of a differential count the DNA distribution was corrected for the content of leucocytes. The DNA histograms were divided into a 2c fraction containing nuclei with less than 3c DNA, a 4c fraction with 3c--6c DNA and an 8c fraction with more than 6c DNA. Three groups of patients were investigated: 17 patients with invasive bladder tumours, 38 patients with non-invasive bladder tumours, and 22 patients with normal bladders. The washings from patients with invasive tumours contained significantly larger 4c and 8c fractions than washings from normal bladders. There was a correlation between cell anaplasia and the DNA distribution: grade III bladder tumours had larger 4c and 8c fractions than grade II tumours.
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Authors | T Pedersen, J K Larsen, T Krarup |
Journal | European urology
(Eur Urol)
Vol. 4
Issue 5
Pg. 351-5
( 1978)
ISSN: 0302-2838 [Print] Switzerland |
PMID | 710467
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Cell Nucleus
(analysis)
- Cytodiagnosis
- DNA, Neoplasm
(analysis)
- Epithelium
(pathology)
- Humans
- Therapeutic Irrigation
- Urinary Bladder
- Urinary Bladder Neoplasms
(diagnosis)
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