Abstract |
Rats were maintained on fat-free high carbohydrate diets either with or without orotic acid (1%, w/w), pantethine (1%, w/w), adenine (0.25%, w/w), and/or p-chlorophenoxyisobutyrate (0.25%, w/w). Oxidation of fatty acid by liver mitochondria was inhibited to less than half that of the control after administration of orotic acid. Activities of acyl-CoA dehydrogenases were markedly decreased by orotic acid administration, but the following enzyme activities were not, or only slightly decreased: acyl-CoA synthetase, carnitine acyltransferases, enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase and 3-ketoacyl-CoA thiolase. Simultaneous addition of pantethine in the orotic acid-containing diet prevented induction of fatty liver. It also prevented decreases in fatty acid oxidation capacity and acyl-CoA dehydrogenase activity. Introduction of adenine or p-chlorophenoxyisobutyrate, which reverse orotic acid-induced fatty liver, reversed oxidation and acyl-CoA dehydrogenase activities to control levels. The oxidation capacity of the peroxisomal system remained unchanged after administration of orotic acid.
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Authors | S Miyazawa, S Furuta, T Hashimoto |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 711
Issue 3
Pg. 494-502
(Jun 11 1982)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 7104378
(Publication Type: Journal Article)
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Chemical References |
- Fatty Acids
- Pantetheine
- Clofibric Acid
- Orotic Acid
- Acyl-CoA Dehydrogenases
- Adenine
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Topics |
- Acyl-CoA Dehydrogenases
(antagonists & inhibitors)
- Adenine
(administration & dosage)
- Animals
- Clofibric Acid
(administration & dosage)
- Diet
- Fatty Acids
(metabolism)
- Fatty Liver
(chemically induced, prevention & control)
- Male
- Mitochondria, Liver
(metabolism)
- Orotic Acid
(administration & dosage)
- Oxidation-Reduction
- Pantetheine
(administration & dosage)
- Rats
- Rats, Inbred Strains
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