HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Assay of leucine aminotransferase in rat tissues and tumors.

Abstract
A survey of leucine aminotransferase activities in an extensive variety of rat tissues further defined the unique distribution of this enzyme. Its reaction was measured in cell-free extracts under optimal conditions, and its product assayed both colorimetrically and with 14CO2 formed from it by decarboxylation with H2O2. The two methods agreed, though the second was more precise and dependable. In all the tissues and cell fractions examined, except adult liver, the activities with valine and isoleucine were parallel and similar to that with leucine. In adult liver there was low activity only with leucine, which is referable to a known isozyme (type II). Although the fraction of enzyme activity present in mitochondria has often been neglected, these studies showed that this portion in all tissues is about equal, more or less, to that of the more familiar soluble forms. There was good agreement between the relative concentrations of this enzyme in the commonly measured rat tissue preparations as determined here and in the more recently published studies. The unique occurrence of high levels of this enzyme in heart, kidney, brain and muscle, in that order, was confirmed. The unusual distribution of this enzyme was emphasized by the recognition that equally high or even higher levels also occurred in pancreas, lactating mammary gland and salivary gland. The relative amounts were less in normal, undifferentiated and in neoplastic tissues.
AuthorsC C Cappuccino, H Kadowaki, W E Knox
JournalEnzyme (Enzyme) Vol. 23 Issue 5 Pg. 328-38 ( 1978) ISSN: 0013-9432 [Print] Switzerland
PMID710384 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Amino Acids, Branched-Chain
  • Ketoglutaric Acids
  • Carbon Dioxide
  • Transaminases
  • Leucine
Topics
  • Amino Acids, Branched-Chain (metabolism)
  • Animals
  • Carbon Dioxide (metabolism)
  • Female
  • In Vitro Techniques
  • Ketoglutaric Acids
  • Leucine
  • Neoplasms (enzymology)
  • Pregnancy
  • Rats
  • Rats, Inbred Strains
  • Spectrophotometry
  • Transaminases (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: