Filter paper strips soaked in 3H-glycine
solution were applied to acoustic cortex of cats, anaesthetized with
Nembutal and pretreated with epileptogenic agents (Metrazol, G-penicillin, and 3-amino-pyridine) and
cycloheximide. The untreated contralateral hemisphere served as control. After 1 h incubation, both cortical samples were excised simultaneously and fixed in Bouin
solution for autoradiography. Incorporation was blocked by
cycloheximide. There was no
glycine incorporation on the
penicillin-treated side, while pyramidal cells were intensively labelled in layers II-V of the mirror focus.
3-Aminopyridine produced the same result.
Metrazol as
convulsant proved to be far weaker than the previous two. The intensity of incorporation was significantly more intensive in the mirror focus than in the primary one.
Penicillin and
3-aminopyridine, while provoking cortical
seizures, seem to inhibit
glycine incorporation into a neuron-specific, function-dependent
protein contained by the labelled cells in the autoradiogram.