Abstract |
The hybridoma technique was used to produce an allotype-specific monoclonal antibody (F11) that reacts with the products of the S, I, and D alleles of PLAP but not of the F allele. Serum and ascites samples from patients with different cancers containing high levels of PLAP were tested for reactivity with F11. These tumor-derived PLAPs were of the Nagao type as shown by their sensitivity to inhibition by L-leucine. This type of inhibition is exhibited also by the rare D allelic variant of PLAP but not by the common forms. Thus, it has been proposed that the Nagao enzyme represents reexpression of the D allele of PLAP. F11 reactive and nonreactive samples as well as samples with intermediate reactivity were found among the cancer sera and ascites. Our results show tha tumor-derived Nagao enzyme does not represent the reexpression of the D allele but instead, in spite of its distinct inhibition pattern, expresses the same genetic polymorphism that is found in the placenta.
|
Authors | J L Millán, T Stigbrand, E Ruoslahti, W H Fishman |
Journal | Cancer research
(Cancer Res)
Vol. 42
Issue 6
Pg. 2444-9
(Jun 1982)
ISSN: 0008-5472 [Print] United States |
PMID | 7074621
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- Antibodies, Monoclonal
- Phenylalanine
- Alkaline Phosphatase
- Leucine
|
Topics |
- Alkaline Phosphatase
(genetics, metabolism)
- Alleles
- Animals
- Antibodies, Monoclonal
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Hybridomas
(immunology)
- Kinetics
- Leucine
(pharmacology)
- Mice
- Mice, Inbred BALB C
- Neoplasms
(enzymology)
- Phenotype
- Phenylalanine
(pharmacology)
- Placenta
(enzymology)
- Polymorphism, Genetic
- Pregnancy
|