A mixture of synthetic L-
amino acids (Aminoplex 14) was infused intravenously over 4 hours in five dogs each prepared with a
gastric fistula and a denervated fundic pouch.
Acid and
pepsin outputs from the innervated fundic pouch.
Acid and
pepsin outputs from the innervated gastric mucosa (
gastric fistula) increased significantly after 90 minutes' infusion of
amino acid.
Acid output from the denervated fundic mucosa (Heidenhain pouch) was estimated by a method of intragastric titration and increased significantly only in response to intravenous
amino acid infusion when the pouch was subjected to distention with 100 ml of perfusate.
Blood glucose, serum
gastrin, hematocrit and serum osmolality levels did not change throughout the experiments. Bolus
intravenous injection of either
atropine (0.15 mg/kg) or
cimetidine (10 mg/kg) blocked the
acid secretory response to intravenous
amino acids. It is concluded that intravenous
amino acids stimulate the innervated gastric mucosa to secrete both
acid and
pepsin. The secretory response from denervated mucosa in only obtained in the presence of fundic pouch distention. The gastric secretory response is susceptible to both
atropine and
histamine H2 antagonist blockade.