Effect of beta-glucuronidase inhibitor on azoxymethane-induced colonic carcinogenesis in rats.

Abstract

In this study of the role of microfloral beta-glucuronidase in colonic carcinogenesis, the effect of beta-glucuronidase inhibitor was evaluated. Starting at 5 weeks of age, male Donryu rats were fed either a semisynthetic diet or the same diet containing 0.1% beta-glucuronidase inhibitor as N-cyclohexyl-5-O-acetyl-2,4-O-p-methoxybenzylidene) -D-glucaro-1-amide-6,3-lactone (C-GAL). All animals were given s.c. injections of 7.4 mg azoxymethane (AOM) per kg body weight once a week for 11 weeks and followed for an additional 20 weeks. Most animals receiving the colonic carcinogen developed tumors in the colon, and a few also developed tumors in the small intestine. However, the number of tumors in the large intestine of the rats given C-GAL at the same time as AOM was significantly lower than in the control rats, especially in the proximal half of the colon, but those given C-GAL after AOM treatment had almost the same number of colon tumors as did the controls. It is concluded that, since bacterial beta-glucuronidase activity in the feces of rats given 0.1% C-GAL was significantly inhibited, intestinal microfloral beta-glucuronidase may play an important role in colonic carcinogenesis caused by AOM.

• Authors: H Takada, T Hirooka, Y Hiramatsu, M Yamamoto
• Journal: Cancer research (Cancer Res) Vol. 42 Issue 1 Pg. 331-34 (Jan 1982) ISSN: 0008-5472 [Print] United States
• PMID: 7053860 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Azo Compounds
• Benzylidene Compounds
• N-cyclohexyl-5-O-acetyl-2,4-O-(4-methoxybenzylidene)glucaro-1-amide-6,3-lactone
• Glucuronidase
• Azoxymethane

Topics

• Animals
• Azo Compounds (metabolism)
• Azoxymethane (metabolism)
• Benzylidene Compounds (pharmacology)
• Biotransformation (drug effects)
• Cocarcinogenesis
• Colonic Neoplasms (chemically induced, enzymology)
• Glucuronidase (antagonists & inhibitors)
• Intestinal Mucosa (microbiology)
• Neoplasms, Experimental (chemically induced, enzymology)
• Rats