HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae.

Abstract
The methods of centrifugal elutriation, two-dimensional gel electrophoresis, and dual isotopic labeling were applied to the study and identification of a number of purified yeast proteins. The location of polypeptide spots corresponding to specific proteins was determined on two-dimensional gels. A dual-label method was used to determine the rates of synthesis through the cell cycle of the identified proteins as well as to confirm the results of previous studies from our laboratory on unidentified proteins. The identified proteins, and the more generally defined phosphorylated, heat shock, and heat stroke proteins were found to follow the general pattern of exponential increase in rate of synthesis through the cell cycle. In addition, colorimetric enzyme activity assays were used to examine the catabolic enzyme alpha-glucosidase (EC 3.2.1.20). Both the activity and synthesis of alpha-glucosidase were found to be nonperiodic with respect to the cell cycle. These data contrast with earlier reports of periodicity, which employed induction and selection synchrony to study enzyme expression through the yeast cell cycle.
AuthorsJ R Ludwig 2nd, J J Foy, S G Elliott, C S McLaughlin
JournalMolecular and cellular biology (Mol Cell Biol) Vol. 2 Issue 2 Pg. 117-26 (Feb 1982) ISSN: 0270-7306 [Print] UNITED STATES
PMID7050667 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Fungal Proteins
  • Heat-Shock Proteins
  • Phosphoproteins
  • Proteins
  • heat stroke proteins
  • alpha-Glucosidases
Topics
  • Cell Cycle
  • Cell Separation
  • Electrophoresis, Polyacrylamide Gel
  • Fungal Proteins (biosynthesis)
  • Heat-Shock Proteins
  • Phosphoproteins (biosynthesis)
  • Protein Biosynthesis
  • Proteins
  • Saccharomyces cerevisiae (metabolism)
  • alpha-Glucosidases (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: