Plasmid-mediated antimicrobial resistance in Haemophilus pleuropneumoniae.

Abstract

Haemophilus pleuropneumoniae, isolated from the lungs of pig, was resistant to ampicillin, streptomycin, and sulfadiazine. This isolate, SD-1, possessed 1 plasmid (pVM105) coded for resistance to ampicillin and sulfadiazine, and the other (pVM104) for resistance to streptomycin and sulfadiazine. A 2nd isolate of H pleuropneumoniae, SD-2, recovered from a pig in a different outbreak of porcine pleuropneumonia, was resistant to streptomycin and sulfadiazine. These resistance determinants were on 1 plasmid (pVM106). All 3 patients were nontransmissible and small (pVM105 = 3.6 x 10(6) daltons, pVM104 and pVM106 = 2.3 x 10(6) daltons. The basis for resistance to ampicillin, encoded on plasmid pVM105, was due to a beta-lactamase. This beta-lactamase was highly active on penicillin G and ampicillin, moderately active on cephalothin, and inactive on oxacillin, indicating a TEM type of beta-lactamase.

• Authors: D C Hirsh, L D Martin, M C Libal
• Journal: American journal of veterinary research (Am J Vet Res) Vol. 43 Issue 2 Pg. 269-72 (Feb 1982) ISSN: 0002-9645 [Print] United States
• PMID: 7046533 (Publication Type: Journal Article)

Chemical References

• Anti-Bacterial Agents
• Sulfadiazine
• Ampicillin
• Streptomycin

Topics

• Ampicillin (pharmacology)
• Animals
• Anti-Bacterial Agents (pharmacology)
• Conjugation, Genetic
• Disease Outbreaks (veterinary)
• Escherichia coli (genetics)
• Haemophilus (drug effects, genetics)
• Haemophilus Infections (microbiology, veterinary)
• Mycoplasma Infections (veterinary)
• Pleuropneumonia, Contagious (microbiology)
• R Factors
• Streptomycin (pharmacology)
• Sulfadiazine (pharmacology)
• Swine
• Swine Diseases (microbiology)
• Transformation, Bacterial