Abstract |
Examined by flow cytofluorometric analysis, the DNA distribution of EMT 6 tumor cells was highly perturbed after one hour of in vitro incubation with: RPCNU, RFCNU, chlorozotocin (CZT) or 185 ( CNCC), four new nitrosourea derivatives. After the treatment with chlorozotocin (20 micrograms/ml) and CNCC (50 micrograms/ml), most of cells were in G2 + M phase and this accumulation lasted more than 48 hours without any restoration before 72 hours. RPCNU (20 micrograms/ml) and RFCNU (50 and 65 micrograms/ml) induced and accumulation of cells in G2 + M phase during 24 hours. The normal state was regained after 48 hours. These reduced rate of progression of the cells through S phase and the G2 block observed after exposure to the new compounds, should, in part, explain their antitumoral activity.
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Authors | M N Vlaeminck, M Collyn-d'Hooghe, P Cappelaere, G Biserte, J Oiry, J L Montero, J L Imbach |
Journal | Biomedicine / [publiee pour l'A.A.I.C.I.G.]
(Biomedicine)
Vol. 35
Issue 1
Pg. 27-9
(Mar 1981)
ISSN: 0300-0893 [Print] France |
PMID | 7016209
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Nitrosourea Compounds
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Topics |
- Antineoplastic Agents
(pharmacology)
- Cell Division
(drug effects)
- Cells, Cultured
- Cytological Techniques
- Fluorometry
- Humans
- Neoplasms, Experimental
(pathology)
- Nitrosourea Compounds
(pharmacology)
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