Abstract |
The microsomal fraction was isolated from homogenate of 125I-labeled leukemia L 1210 ascites cells by filtration of postmitochondrial supernatant through a Sepharose 4 B column. It was found that the particles are labeled with iodine and show 5'-nucleotidase activity suggesting the presence of cell membranes in the fraction. The soluble proteins fraction were retarded on the column showed lactate dehydrogenase activity, and low activity of soluble beta-D- glucuronidase.
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Authors | B Miks, J Kawiak, G Hoser |
Journal | Folia histochemica et cytochemica
(Folia Histochem Cytochem (Krakow))
Vol. 16
Issue 3
Pg. 187-92
( 1978)
ISSN: 0015-5586 [Print] Poland |
PMID | 700505
(Publication Type: Journal Article)
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Chemical References |
- Iodine Radioisotopes
- L-Lactate Dehydrogenase
- Nucleotidases
- Glucuronidase
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Topics |
- Cell Line
- Chromatography, Gel
- Glucuronidase
(metabolism)
- Iodine Radioisotopes
- L-Lactate Dehydrogenase
(metabolism)
- Leukemia, Lymphoid
- Microsomes
(enzymology)
- Nucleotidases
(metabolism)
- Subcellular Fractions
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