Abstract |
Interleukin 2 (IL-2), produced with and without co-stimulation by the Burkitt's lymphoma line Daudi, was purified 37,000-fold to apparent homogeneity from lymphocyte conditioned medium by (NH4)2SO4 precipitation, DEAE-cellulose ion-exchange chromatography, gel filtration, and chromatography on blue agarose and on Procion-red agarose. The purified IL-2 showed a 10(6) U/mg protein sp act. IL-2 produced in the absence of Daudi cells had a mol wt of 26,000 as measured by gel filtration and an isoelectric point of 6.7. This IL-2 showed a 16,000 and 17,000 mol wt in sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE). IL-2, produced in the presence of Daudi cells (10(6)/ml), showed a mol wt of approximately 14,000, as measured by both gel filtration and SDS-PAGE, and an isoelectric point of 8.1. The purified IL-2 lacked detectable interferon (alpha and gamma), granulocyte-macrophage colony-stimulating factor, B cell growth factor, T cell-replacing factor, and thymocyte-differentiating activity and was free of any contaminating proteins as judged by silver staining in SDS-PAGE. All three molecular forms of IL-2 were biologically active at concentrations of 10(-11) - 10(-10) M, supporting the growth of human and murine cytotoxic T cell lines.
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Authors | K Welte, C Y Wang, R Mertelsmann, S Venuta, S P Feldman, M A Moore |
Journal | The Journal of experimental medicine
(J Exp Med)
Vol. 156
Issue 2
Pg. 454-64
(Aug 01 1982)
ISSN: 0022-1007 [Print] United States |
PMID | 6980256
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Interleukin-2
- Lymphokines
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Topics |
- Animals
- Biological Assay
- Cell Division
(drug effects)
- Cell Line
- Electrophoresis, Polyacrylamide Gel
- Humans
- Interleukin-2
(isolation & purification, pharmacology)
- Isoelectric Focusing
- Lymphokines
(isolation & purification)
- Mice
- Molecular Weight
- T-Lymphocytes
(drug effects, immunology)
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