Lack of expression on cultured human T-lymphocytes of a T-cell antigen shared by the MOLT-4 cell line and normal human thymocytes.

Abstract

Four hematopoietic cell lines (CCRF-CEM, HSB-2, MOLT-4, and RPMI-8402), derived from acute lymphoblastic leukemia and expressing T-cell surface markers (T-HCL), were studied with two specific anti-T-cell sera. The sera were raised in rabbits against human thymocytes (anti-HTY) and against T-cell cultured in the presence of conditioned medium derived from lymphocytes stimulated with PHA (anti-CTC). Both sera were absorbed to obtain a T-cell specific pattern of reaction and were further absorbed with normal peripheral blood lymphocytes or with each of the four T-HCL. The anti-HTY sera absorbed with CEM, 8402, and HSB-2 still reacted with MOLT-4. A similar pattern of reactivity was found only with the anti-CTC absorbed with 8402, whereas, after absorptions with the other cell lines, this antiserum was unreactive against MOLT-4. After absorption with normal peripheral blood lymphocytes, anti-HTY still reacted with thymocytes and MOLT-4 but was negative on CTC. In contrast, anti-CTC absorbed with peripheral blood lymphocytes (PBL) was negative on thymocytes and MOLT-4 but still reacted against CTC. Our data confirm the existence of a T-cell antigen (probably an early T-cell differentiation antigen) shared between thymus and MOLT-4. This antigen is not expressed on CTC, although these cells express an antigenic pattern more complex than PBL. Antisera to CTC represents a source of anti-T cell sera free of contamination with antibodies to early thymus-related antigens but containing other T-cell-related specificities.

• Authors: F Pandolfi, D M Strong, G D Bonnard, R B Herberman
• Journal: In vitro (In Vitro) Vol. 16 Issue 9 Pg. 754-60 (Sep 1980) ISSN: 0073-5655 [Print] United States
• PMID: 6967850 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Surface
• Antilymphocyte Serum

Topics

• Antigens, Surface (analysis)
• Antilymphocyte Serum
• Cell Line
• Cells, Cultured
• Leukemia, Lymphoid
• T-Lymphocytes (immunology)