Classification of normal and malignant lymphatic cells using acid phosphatase and acid esterase.

Abstract

UNLABELLED:

The usefulness of cytochemical tests (APh and ANAE) to replace or to supplement membrane markers in subclassification of normal and malignant lymphatic cells was investigated.

MATERIAL:

normal lymphocytes subfractionated by rosetting and centrifugation, and in M. Hodgkin and CLL; lymphoblastoid cell lines; malignant lymphatic cells in different types of lymphatic leukemia. In normal human blood, T-lymphocytes are marked by a distinct "dot-like" ANAE-reactivity which is somewhat less pronounced in the small (11%) subgroup of Fc-IgG-receptor positive T-lymphocytes; B-lymphocytes are negative or finely granular positive. Lymphoblastoid cell lines of B- and of T-type are ANAE- and APh-positive. In some lymphatic malignancies, a characteristic pattern of activity of APh or of ANAE may support the diagnosis. The value of ANAE-cytochemistry is highly estimated for the quantitative determination of the percentage of normal T-lymphocytes lymphatic leukemias, immunological disorders, and during immunosuppressive therapy.

• Authors: D Huhn, E Thiel, H Rodt
• Journal: Klinische Wochenschrift (Klin Wochenschr) Vol. 58 Issue 2 Pg. 65-71 (Jan 15 1980) ISSN: 0023-2173 [Print] Germany
• PMID: 6966011 (Publication Type: Journal Article)

Chemical References

• Esterases
• Acid Phosphatase

Topics

• Acid Phosphatase
• B-Lymphocytes (classification)
• Esterases
• Hodgkin Disease (pathology)
• Humans
• Leukemia, Lymphoid (pathology)
• Lymphocytes (classification)
• T-Lymphocytes (classification)