Immunization with R mutants of Salmonella minnesota. II. Serological response to lipid A and the lipopolysaccharide of Re mutants.

Abstract

Rabbits were immunized with Salmonella minnesota Re mutant bacilli or lipid A, and the serological responses were compared. Re lipopolysaccharide and lipid A were found to possess distinct antigenic determinants as measured by hemagglutination, hemolysis, and precipitin techniques. Lipid A preparations from several enterobacterial and non-enterobacterial species were shown to cross-react in agar gel precipitation reactions. In contrast, preparations from bacilli that do not contain glucosamine as an integral part of their lipid A structure did not demonstrate cross-reactivity with enterobacterial lipid A. Antibody to smooth heterologous bacilli present in some antisera prepared against Re bacilli or lipid A was directed specifically against the smooth lipopolysaccharide and not against the Re or lipid A determinants, demonstrating a nonspecific mitogenic response. Precipitation and immunofluorescence tests indicated that the Re determinant was available on smooth bacilli for reactions with specific antisera but that the lipid A determinant was not.

• Authors: M A Johns, S C Bruins, W R McCabe
• Journal: Infection and immunity (Infect Immun) Vol. 17 Issue 1 Pg. 9-15 (Jul 1977) ISSN: 0019-9567 [Print] United States
• PMID: 69613 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Epitopes
• Lipids
• Lipopolysaccharides
• Polysaccharides, Bacterial

Topics

• Animals
• Antibodies, Bacterial (biosynthesis)
• Antigens, Bacterial
• Cross Reactions
• Enterobacteriaceae (immunology)
• Epitopes
• Immunization
• Lipids (immunology)
• Lipopolysaccharides (immunology)
• Mutation
• Polysaccharides, Bacterial (immunology)
• Pseudomonas aeruginosa (immunology)
• Rabbits
• Salmonella (immunology)