N,N-dimethylformamide-induced modulation of organ- and tumor-associated markers in cultured human colon carcinoma cells.

Abstract

Cultured human colon carcinoma cells were induced by the polar solvent N,N-dimethylformamide (DMF) to express a more differentiated phenotype as indicated by three types of antigen markers. Carcinoembryonic antigen expression in all DMF-induced cell lines was enhanced. Exposure of cells to DMF resulted in a reduction in ability to bind antibody to tumor-derived colon mucoprotein antigen (CMA) with a concomitant gain in binding of antibody to normal CMA. DMF-induced differentiation was further indicated by modulation of blood group antigen expression. DMF treatment decreased the amount of expression of the H-gene determinant. When DMF-treated cells were cultured in the absence of DMF for 14 days, the levels of expression of the antigen markers reverted to those characteristic of their untreated counterparts.

• Authors: J C Hager, D V Gold, J A Barbosa, Z Fligiel, F Miller, D L Dexter
• Journal: Journal of the National Cancer Institute (J Natl Cancer Inst) Vol. 64 Issue 3 Pg. 439-46 (Mar 1980) ISSN: 0027-8874 [Print] United States
• PMID: 6928233 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• ABO Blood-Group System
• Antigens
• Carcinoembryonic Antigen
• Mucoproteins
• Dimethylformamide

Topics

• ABO Blood-Group System
• Adenocarcinoma (drug therapy)
• Antigens
• Carcinoembryonic Antigen
• Cell Differentiation (drug effects)
• Cell Line
• Colon (immunology)
• Colonic Neoplasms (drug therapy, immunology)
• Dimethylformamide (pharmacology)
• Humans
• Mucoproteins (immunology)
• Rosette Formation