HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Incorporation rates of [1-14C]glycerol into the molecular species of alkyl ether phospholipids of Ehrlich ascites tumor cells in vivo.

Abstract
[1-14C]Glycerol was injected into the peritoneal cavity of mice bearing Ehrlich ascites tumor cells and the lipids were extracted from the cells after selected periods. 1-O-Alkyl-2-acyl-3-acetyl-glycerols and 1,2-diacyl-3-acetylglycerols were prepared from the ethanolamine and choline phosphoglycerides and fractionated by AgNO3-impregnated thin-layer chromatography into seven molecular species. Bands 1--7 obtained in this way can be designed as saturated, monoene, diene (11), diene (02), triene, tetraene and pentaene + hexaene species, respectively. Band 7, composed mainly of hexaene species, was predominant among those derived from ether phospholipids. The predominant species was band 4 (saturated/linoleate) in both diacyl ethanolamine and choline phospholipids. The specific radioactivities of the fractionated species were determined. In diacyl or alkyl ether compounds of ethanolamine and choline phosphoglycerides, disaturated species turned over very rapidly, while in alkyl ether ethanolamine phosphglyceride, hexaene + pentaene molecular species turned over rapidly. In diacyl compounds of the ethanolamine phosphoglyceride, hexaene molecular species also turned over at a high rate. In diacyl ethanolamine or choline phosphoglycerides, dienoic species turned over rather slowly.
AuthorsK Waku, Y Nakazawa
JournalEuropean journal of biochemistry (Eur J Biochem) Vol. 88 Issue 2 Pg. 489-94 (Aug 01 1978) ISSN: 0014-2956 [Print] England
PMID689034 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Fatty Acids
  • Phosphatidylcholines
  • Phosphatidylethanolamines
  • Phospholipids
  • Glycerol
Topics
  • Animals
  • Carcinoma, Ehrlich Tumor (metabolism)
  • Fatty Acids (analysis)
  • Glycerol (metabolism)
  • Male
  • Mice
  • Phosphatidylcholines (biosynthesis)
  • Phosphatidylethanolamines (biosynthesis)
  • Phospholipids (biosynthesis)
  • Structure-Activity Relationship

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: