[1-14C]
Glycerol was injected into the peritoneal cavity of mice bearing
Ehrlich ascites tumor cells and the
lipids were extracted from the cells after selected periods. 1-O-Alkyl-2-acyl-3-acetyl-glycerols and 1,2-diacyl-3-acetylglycerols were prepared from the
ethanolamine and
choline phosphoglycerides and fractionated by AgNO3-impregnated thin-layer chromatography into seven molecular species. Bands 1--7 obtained in this way can be designed as saturated, monoene, diene (11), diene (02), triene, tetraene and pentaene + hexaene species, respectively. Band 7, composed mainly of hexaene species, was predominant among those derived from
ether phospholipids. The predominant species was band 4 (saturated/
linoleate) in both diacyl
ethanolamine and
choline phospholipids. The specific radioactivities of the fractionated species were determined. In diacyl or alkyl
ether compounds of
ethanolamine and
choline phosphoglycerides, disaturated species turned over very rapidly, while in alkyl
ether ethanolamine phosphglyceride, hexaene + pentaene molecular species turned over rapidly. In diacyl compounds of the
ethanolamine phosphoglyceride, hexaene molecular species also turned over at a high rate. In diacyl
ethanolamine or
choline phosphoglycerides, dienoic species turned over rather slowly.