Abstract |
To evaluate the usefulness of plasmid pattern analysis as an epidemiologic tool and to determine the mechanism of resistance to silver sulfadiazine and mafenide acetate, silver sulfadiazine-resistant, mafenide acetate-resistant Enterobacter cloacae isolates from a 1976 outbreak of infections in a burn unit (group I) were analyzed retrospectively. These strains were compared with various E cloacae isolates (groups II-V), including E cloacae isolates from a more recent (1982) burn unit outbreak (group V). Agarose gel electrophoresis (AGE) of DNA isolated from "epidemic" strains of E cloacae revealed a uniform pattern of four plasmid DNA bands, ranging in molecular size from 66 to 2 megadaltons. No such plasmid pattern was seen in silver sulfadiazine-, mafenide acetate-susceptible isolates of E cloacae in groups II, III, and V. Analysis of non-E cloacae coisolates suggested that silver sulfadiazine resistance was associated with the 55-megadalton plasmid. Plasmid pattern analysis easily separated "epidemic" E cloacae isolated in 1976 from those isolated in 1982.
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Authors | S M Markowitz, S M Smith, D S Williams |
Journal | The Journal of infectious diseases
(J Infect Dis)
Vol. 148
Issue 1
Pg. 18-23
(Jul 1983)
ISSN: 0022-1899 [Print] United States |
PMID | 6886483
(Publication Type: Journal Article)
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Topics |
- Burns
(complications, microbiology)
- Disease Outbreaks
(epidemiology)
- Drug Resistance, Microbial
- Electrophoresis, Agar Gel
- Enterobacter
(genetics, isolation & purification)
- Enterobacteriaceae Infections
(complications, epidemiology, microbiology)
- Humans
- Plasmids
(drug effects)
- Retrospective Studies
- Sepsis
(etiology, microbiology)
- Virginia
- Wound Infection
(etiology, microbiology)
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