Lethal and cytokinetic effects of anguidine on a human colon cancer cell line.

Abstract

Anguidine is a fungal metabolite with antitumor activity in a murine colon cancer model. Because of disappointing results in clinical trials, we analyzed the lethal and cytokinetic effects of anguidine on cultured human colon cancer cells. The studies revealed a moderate reduction in survival only after prolonged drug exposure. Continuous incubation with anguidine for longer than 48 hr produced a moderate increase in the percentage of S-phase cells and a slight decrease in the proportion of cells in G1/0, by pulse cytophotometry. An immediate reduction in the cumulative labeling index for cells continuously exposed to tritiated thymidine and anguidine and a rapid decrease in the cumulative mitotic index for cells continuously exposed to Colcemid and anguidine indicated a block at the G1 into S and G2 into mitosis transitions. Tumoricidal activity of anguidine in a cultured human colon cancer line is poor and requires prolonged exposure. The kinetic data reflect an almost frozen state of the cell cycle.

• Authors: G M Dosik, B Barlogie, D A Johnston, W K Murphy, B Drewinko
• Journal: Cancer research (Cancer Res) Vol. 38 Issue 10 Pg. 3304-9 (Oct 1978) ISSN: 0008-5472 [Print] United States
• PMID: 688220 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Sesquiterpenes
• Trichothecenes
• diacetoxyscirpenol

Topics

• Adenocarcinoma (drug therapy, pathology)
• Cell Cycle (drug effects)
• Cell Line
• Cell Survival (drug effects)
• Colonic Neoplasms (drug therapy, pathology)
• Humans
• Kinetics
• Neoplasms, Experimental (drug therapy)
• Sesquiterpenes (pharmacology)
• Trichothecenes (administration & dosage, pharmacology)