Decrease in the levels of specific non-polysomal messenger ribonucleoproteins during the mouse sarcoma-180 ascites cell cycle.

Abstract

A few mRNAs in S-180 ascites cells are found as non-polysomal messenger ribonucleoprotein particles, whose translation is restricted both in vivo and in vitro. To determine if these mRNPs remain untranslated throughout the cell cycle we obtained S-180 ascites cells enriched in cell cycle stages by centrifugation through ficoll gradients. The "inactive" mRNA species were clearly present in the pre-polysomal fraction from S and pre-S phase cells, however they were not detected in the mRNP fraction from post S phase cells. Hybridization of a cloned cDNA probe for one of these species (P-40) to polysomal RNA from pre- or post-S phase cells demonstrated it was present in substantially higher concentrations in polysomes from post-S phase cells, consistent with its translation after S phase. We also observed that a poly (A)-minus form of the mRNA coding for actin was restricted to post S phase cells.

• Authors: T E Geoghegan, S J Knoop, C Chitpatima
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 113 Issue 3 Pg. 923-33 (Jun 29 1983) ISSN: 0006-291X [Print] United States
• PMID: 6870902 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Nucleoproteins
• RNA, Messenger
• RNA, Neoplasm
• Ribonucleoproteins

Topics

• Animals
• Ascitic Fluid (metabolism)
• Cell Cycle
• Cell-Free System
• In Vitro Techniques
• Mice
• Nucleoproteins (metabolism)
• Polyribosomes (metabolism)
• Protein Biosynthesis
• RNA, Messenger (isolation & purification)
• RNA, Neoplasm (metabolism)
• Ribonucleoproteins (metabolism)
• Sarcoma 180 (metabolism)