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Studies on demyelination in vitro: the requirement of membrane attack components of the complement system.

Abstract
Anti-spinal cord antibodies (anti-SC) cause demyelination of well myelinated mouse cerebellum cultures in the presence of fresh serum. Heating the serum for 30 min at 56 degrees C abolishes the demyelinating activity. We studied the role of complement (c) in demyelination initiated by anti-SC in well myelinated mouse cerebellum cultures. Demyelination was assessed morphologically. The extent of demyelination was correlated to the dose of whole serum C as well as the dose of antibody. To evaluate the requirement of membrane attack components of C, C5b-C9, sister cultures were treated with antibody + C8 deficient human serum (C8D-HS) with and without purified human C8. Extensive demyelination was observed in C8-reconstituted cultures whereas antibody + C8D-HS did not demyelinate, indicating the essential requirement of C5b-8, and/or C5b-9. Extensively demyelinated cultures remyelinated when fresh medium was supplied, suggesting that the process of antibody and C-mediated demyelination is selective for myelin membrane in this system.
AuthorsW T Liu, P Vanguri, M L Shin
JournalJournal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 131 Issue 2 Pg. 778-82 (Aug 1983) ISSN: 0022-1767 [Print] United States
PMID6863930 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Antibodies
  • Complement Membrane Attack Complex
  • Culture Media
  • Complement System Proteins
Topics
  • Animals
  • Antibodies (immunology)
  • Cerebellum (ultrastructure)
  • Complement Membrane Attack Complex
  • Complement System Proteins (immunology)
  • Culture Media
  • Hot Temperature
  • Mice
  • Myelin Sheath (immunology)
  • Spinal Cord (immunology)

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