Increased fraction of acid-soluble proteins in 0.35 M NaCl extracts of nuclei from rat liver tumors.

Abstract

1. The fraction of proteins extracted from nuclei with 0.35 M NaCl and soluble in 2% trichloroacetic acid was examined in five Morris hepatomas and rat liver. 2. This fraction was a much greater percentage of the total 0.35 M NaCl soluble proteins in the tumors than in normal or regenerating liver. 3. In part, this difference was due to proteins with molecular weights greater than high mobility group proteins. 4. The conditions for precipitation of high mobility group proteins 1 and 2 with trichloroacetic acid were found to differ in hepatoma and liver fractions.

• Authors: M A Lea
• Journal: The International journal of biochemistry (Int J Biochem) Vol. 15 Issue 4 Pg. 513-22 ( 1983) ISSN: 0020-711X [Print] England
• PMID: 6852350 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Neoplasm Proteins
• Nucleoproteins
• Sodium Chloride

Topics

• Animals
• Cell Nucleus (analysis)
• Cytosol (analysis)
• Hydrogen-Ion Concentration
• Liver (analysis)
• Liver Neoplasms, Experimental (analysis)
• Male
• Molecular Weight
• Neoplasm Proteins (isolation & purification)
• Nucleoproteins (isolation & purification)
• Rats
• Rats, Inbred BUF
• Sodium Chloride
• Solubility