Changes in the binding of radioactive conjugated bile salts to serum proteins in cholestatic jaundice.

Abstract

1. A micro-partition centrifugal ultrafiltration technique has been used to monitor the percentage of [14C]glycocholate, [3H]glycochenodeoxycholate and [3H]glycochenodeoxycholate-3-sulphate bound to serum proteins of patients with cholestatic liver disease. 2. In comparison with normal individuals the percentage of binding of [14C]glycocholate and, to a lesser extent, of [3H]glycochenodeoxycholate and [3H]glycochenodeoxycholate-3-sulphate was reduced. 3. The binding of [14C]glycocholate was inversely related to the serum bile salt and bilirubin concentrations. In contrast, the binding of [3H]glycochenodeoxycholate and [3H]glycochenodeoxycholate-3-sulphate were not altered by the severity of the cholestasis. 4. Studies in vitro indicated that the reduction in the binding of [14C]glycocholate in cholestatic liver disease was not due to high concentrations of bile salts, unconjugated bilirubin or fatty acids.

• Authors: A Chitranukroh, B H Billing
• Journal: Clinical science (London, England : 1979) (Clin Sci (Lond)) Vol. 65 Issue 1 Pg. 77-84 (Jul 1983) ISSN: 0143-5221 [Print] England
• PMID: 6851421 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bile Acids and Salts
• Blood Proteins
• Glycochenodeoxycholic Acid
• glycochenodeoxycholate-3-sulfate
• Glycocholic Acid
• Bilirubin

Topics

• Bile Acids and Salts (blood)
• Bilirubin (blood)
• Blood Proteins (metabolism)
• Cholestasis (blood)
• Glycochenodeoxycholic Acid (analogs & derivatives, blood)
• Glycocholic Acid (blood)
• Humans
• Protein Binding