Tumor-imaging potential of liposomes loaded with In-111-NTA: biodistribution in mice.

Abstract

EMT6 tumors in BALB/c mice have been successfully imaged with small (less than 0.1 mu), unilamellar lipid vesicles (SUVs) loaded with In-111 nitrilotriacetic acid (In-111 NTA). Neutral SUVs prepared from distearoyl phosphatidylcholine (DSPC) and cholesterol (CH) (ratio 2:1) delivered sufficient radioactivity to allow tumor visualization 24 hr after i.v. injection; so did positively and negatively charged SUVs with the ratio 4:1:1 for DSPC:CH:X, were X was stearylamine or dicetyl phosphate. Other SUVs containing a 6-aminomannose or 6-aminomannitol derivative of cholesterol did not cause significant tumor accumulation of In-111 NTA, and tumor images were not readily discernible. The maximum tumor-associated radioactivity, 18.5% of injected dose per gram of tissue, was achieved with neutral SUVs. This level of tumor-associated In-111 was over 4 times that observed when unencapsulated In-111 NTA was injected. Neutral SUVs also gave the lowest specific activities in the liver and spleen (14.6% and 18.8% of dose respectively).

• Authors: R T Proffitt, L E Williams, C A Presant, G W Tin, J A Uliana, R C Gamble, J D Baldeschwieler
• Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (J Nucl Med) Vol. 24 Issue 1 Pg. 45-51 (Jan 1983) ISSN: 0161-5505 [Print] United States
• PMID: 6848703 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Acetates
• Liposomes
• Radioisotopes
• Indium
• Nitrilotriacetic Acid

Topics

• Acetates
• Animals
• Cell Line
• Indium
• Liposomes (administration & dosage)
• Male
• Mice
• Mice, Inbred BALB C
• Neoplasm Transplantation
• Neoplasms, Experimental (diagnostic imaging)
• Nitrilotriacetic Acid (metabolism)
• Radioisotopes
• Radionuclide Imaging
• Time Factors
• Tissue Distribution