Abstract |
An in vitro method for culturing Limax maximus albumen glands is described in which the biosynthetic activity of the slug albumen gland was monitored by measuring the incorporation of [14C] glucose into galactogen. Homogenates of the central nervous system were shown to cause a 3.5- to 12-fold increase in galactogen synthesis in albumen gland explants as compared to controls. The major sources of the galactogen-synthesis stimulating factor (Gal-SF) were found to be the cerebral ganglia and their surrounding connective tissue. Gal-SF was demonstrated to be peptidase sensitive and heat labile suggesting that it is probably a polypeptide. Autoradiographs of semithin araldite sections supported the incorporation data: in albumen gland explants cultured with cerebral ganglion homogenate considerably more label was found over secretory granules than in control-cultured explants. The possible cellular source of Gal-SF is discussed in relation to its possible origin in other investigated pulmonates.
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Authors | J van Minnen, J Wijdenes, P G Sokolove |
Journal | General and comparative endocrinology
(Gen Comp Endocrinol)
Vol. 49
Issue 2
Pg. 307-14
(Feb 1983)
ISSN: 0016-6480 [Print] United States |
PMID | 6840525
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Albumins
- Galactans
- Tissue Extracts
- galactogen
- Pronase
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Topics |
- Albumins
(physiology)
- Animals
- Autoradiography
- Central Nervous System
(physiology)
- Endocrine Glands
(physiology)
- Exocrine Glands
(physiology)
- Female
- Galactans
(biosynthesis)
- Mollusca
(metabolism)
- Pronase
- Tissue Extracts
(pharmacology)
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