Differentiation of a histiocytic lymphoma cell line by lipomodulin, a phospholipase inhibitory protein.

Abstract

When U 937 cells, a human histiocytic lymphoma cell line, were cultured with purified lipomodulin for 3 days, morphological and functional differentiation was induced as detected by microscopical examination of Giemsa stained smears, expression of mature monocyte antigen, and antibody dependent cellular cytotoxicity tests. Essentially similar differentiation was observed by the treatment with dexamethasone for 6 days and this differentiation by dexamethasone was blocked by monoclonal anti-lipomodulin antibody. Furthermore, the synthesis of immunoprecipitable lipomodulin in these cells was induced by dexamethasone treatment. These results, taken together, suggest that the induction of lipomodulin synthesis might be the primary event in dexamethasone-induced cellular differentiation of U 937 cells.

• Authors: T Hattori, T Hoffman, F Hirata
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 111 Issue 2 Pg. 551-9 (Mar 16 1983) ISSN: 0006-291X [Print] United States
• PMID: 6838573 (Publication Type: Journal Article)

Chemical References

• Annexins
• Antigens, Surface
• Calcium-Binding Proteins
• Glycoproteins
• Proteins
• lipomodulin
• Dexamethasone

Topics

• Annexins
• Antigens, Surface (analysis)
• Calcium-Binding Proteins
• Cell Differentiation (drug effects)
• Cell Line
• Dexamethasone (pharmacology)
• Glycoproteins
• Humans
• Lymphoma (immunology, pathology)
• Proteins (pharmacology)